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KMID : 0545120010110020287
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Journal of Microbiology and Biotechnology
2001 Volume.11 No. 2 p.287 ~ p.293
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Mutations in the rpoB Gene of Mycobacterium leprae from Korean Leprosy Patients
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Kim, Soon Ok
Chae, Gue Tae/Shin, Hang Kye/Kim, Nan Hee/Lee, In Hyung/Suh, Joo Won
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Abstract
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A fast and easy PCR-SSCP method was developed and assessed for the early detection of rifampin-resistant Mycobacterium leprae in skin biopsy samples from Korean leprosy patients. The 190bp of the rpoB gene, in which mutation is known to cause resistance to rifampin, was amplified by PCR and then analyzed by SSCP and DNA sequencing. All PCR products showing mobility shift on PCRSSCP contained mutations, demonstrating that this method can be used for an early diagnostic method to detect a putative rifampin-resistant M. leprae strain. DNA sequence analysis revealed that 19 of 34 patient samples contained M. leprae strains with missense mutations in the rpoB gene: five were the same mutations previously reported to cause rifampin resistance and eight were the new type of mutations that likely cause rifampin resistance. These newly identified mutations, whose all five cytosine bases of four amino acids were substituted with thymine, were found at different sites from those reported in Mycobacterium tuberculosis or M. leprae. Therefore, they may provide additional clues to understand the molecular biological basis on the rifampin resistance of M. leprae.
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